Ansari, Anees A. and Kaushik, A. and Solanki, P. R. and Malhotra, B. D. (2008) Sol-gel derived nanoporous cerium oxide film for application to cholesterol biosensor. Electrochemistry Communications , 10 (9). 1246-1249 . ISSN 1388-2481

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Abstract

Cholesterol oxidase (ChOx) has been immobilized onto sol-gel derived nano-structured cerium oxide (NS-CeO2) film deposited on indium-tin-oxide (ITO) coated glass substrate. Phase identification of sol-gel NS-CeO2 film carried out using X-ray diffraction (XRD) yields reflection peak at 29.4 degrees corresponding to (111) plane with oriented crystallite (34 nm) along c-axis normal to the substrate. Electrochemical studies reveal that NS-CeO2 provides electroactive surface for the loading of ChOx and enhances electron transfer rate in the ChOx/NS-CeO2/ITO bioelectrocle. The low value of Michaelis-Menten constant (K-m) obtained as 2.08 mM indicates enhanced ChOx affinity to cholesterol. The observed results show application of sol-gel derived NS-CeO2 for biosensing without any functionalization.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier.
Subjects:	Electrochemistry Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	18 Apr 2017 06:54
Last Modified:	18 Apr 2017 06:54
URI:	http://npl.csircentral.net/id/eprint/2106

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