KamilReza, K. and Ali, Md.Azahar and Srivastava, Saurabh and Agrawal, Ved Varun and Biradar, A. M. (2015) Tyrosinase conjugated reduced graphene oxide based biointerface for bisphenol A sensor. Biosensors and Bioelectronics, 74. 644-651 . ISSN 0956-5663

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We have fabricated a nanocomposite of reduced graphene oxide (rGO) sheets and chitosan (Cn) polymer based highly sensitive electrochemical biosensor for detection of bisphenol A (BPA). The two-dimensional structure and chemical functionality of rGO and Cn provide an excellent electrode surface for loading of tyrosinase enzyme molecules. This rGO-Cn nanocomposite is capable of effectively utilizing their superior conductivity, larger effective surface area and superior electrochemical performance due to its synergistic effect between rGO and Cn. The structural, morphological and electrochemical characterizations of nanocomposite sheets have been performed by electron microscopy, X-ray diffraction, FTIR and Potentiostat/Galvanostat techniques. This fabricated biosensor is sensitive to nanomolar (0.74 nM) concentration of BPA and detection time is 10 s compared to conventional BPA ELISA kit (0.3 mu g/L and 2.5 h). The rGO-Cn based biosensor exhibits a higher sensitivity (83.3 mu A nM(-1) cm(-2)), wider linearity (0.01-50 mu M) with good selectivity towards BPA. This biosensor is capable to quantify real sample of BPA using packaged drinking water bottles. This rGO-Cn nanocomposite sheets emerges as a potential electrode material for detection of other estrogenic substrate.

Item Type: Article
Additional Information: Copyright for this article belongs to M/S Elsevier B.V.
Uncontrolled Keywords: Reducedgrapheneoxide Nanocomposite Bisphenol A Tyrosinase Electrochemical sensor
Subjects: Biotechnology & Applied Microbiology
Chemistry > Analytical Chemistry
Nanoscience/ Nanotechnology
Depositing User: Dr. Rajpal Walke
Date Deposited: 30 Jan 2017 12:01
Last Modified: 30 Jan 2017 12:01
URI: http://npl.csircentral.net/id/eprint/2056

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