Singh, Nawab and Reza, K. Kamil and Ali, Md. Azahar and Agrawal, Ved Varun and Biradar, A. M. (2015) Self assembled DC sputtered nanostructured rutile TiO2 platform for bisphenol A detection. Biosensors and Bioelectronics, 68. 633 -641 . ISSN 0956-5663

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Abstract

A novel biosensor platform comprising of the functionalized sputtered rutile nanostructured titanium dioxide (nTiO(2)) for rapid detection of estrogenic substance (bisphenol A) has been proposed. The direct current (DC) sputtering of titanium (Ti) on glass substrate has been converted to ordered nanostructured TiO2 film via oxidation. The nanostructured TiO2 surface was functionalized with self-assembled monolayer (SAM) of 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde. The enzyme molecule, tyrosinase (Tyrs) has been covalently immobilized on the surface of APTES modified nanostructured TiO2 film. To investigate the crystalline structure and surface morphology of functionalized nTiO(2)/Ti electrode, the X-ray diffraction, scanning electron microscopy, atomic force microscopy and Fourier transform infrared spectroscopy have been carried out. This impedimetric biosensor exhibits a comparable sensitivity (361.9 k Omega/mu M) in a wide range of detection (0.01-1.0 mu M) and a response time of 250 s for bisphenol A (BPA) monitoring. This novel manufacturing process for nTiO(2) film is cheap, practical and safer for functionalization with SAM and glutaraldehyde to improve the biosensor efficacy. The strong protein absorption capability of the nTiO(2) surface demonstrates an excellent electrochemical biosensor and could be useful for the detection of other phenolic compounds.

Item Type:	Article
Uncontrolled Keywords:	DC sputtering Electrochemical oxidation Nanocrystalline titanium Nanostructured TiO2 Bisphenol A Tyrosinase APTES
Subjects:	Biophysics Chemistry > Analytical Chemistry Nanoscience/ Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	13 Oct 2016 07:22
Last Modified:	13 Oct 2016 07:22
URI:	http://npl.csircentral.net/id/eprint/1982

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