Ghrera, Aditya Sharma and Pandey, Chandra Mouli and Ali, Md. Azahar and Malhotra, Bansi Dhar (2015) Quantum dot-based microfluidic biosensor for cancer detection. Applied Physics Letters, 106 (19). ISSN 0003-6951

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We report results of the studies relating to fabrication of an impedimetric microfluidic-based nucleic acid sensor for quantification of DNA sequences specific to chronic myelogenous leukemia (CML). The sensor chip is prepared by patterning an indium-tin-oxide (ITO) coated glass substrate via wet chemical etching method followed by sealing with polydimethylsiloxane (PDMS) microchannel for fluid control. The fabricated microfluidic chip comprising of a patterned ITO substrate is modified by depositing cadmium selenide quantum dots (QCdSe) via Langmuir-Blodgett technique. Further, the QCdSe surface has been functionalized with specific DNA probe for CML detection. The probe DNA functionalized QCdSe integrated miniaturized system has been used to monitor target complementary DNA concentration by measuring the interfacial charge transfer resistance via hybridization. The presence of complementary DNA in buffer solution significantly results in decreased electroconductivity of the interface due to presence of a charge barrier for transport of the redox probe ions. The microfluidic DNA biosensor exhibits improved linearity in the concentration range of 10(-15) M to 10(-11) M.

Item Type: Article
Subjects: Applied Physics/Condensed Matter
Depositing User: Dr. Rajpal Walke
Date Deposited: 06 Oct 2016 07:01
Last Modified: 06 Oct 2016 07:01

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