Nirala, Narsingh R. and Abraham, Shiju and Kumar, Vinod and Pandey, Shobhit A. and Yadav, Umakant and Srivastava, Monika and Srivastava, S. K. and Singh, Vidyanand and Kayastha, Arvind M. and Srivastava, Anchal and Saxena, Preeti S. (2015) Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance. Talanta, 144. 745-754 . ISSN 0039-9140

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The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing. Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 mu A/(mg/dL)/cm(2) and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL. Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode. This is evident from one order higher electron transfer rate constant (K-s) value in case of CH-prGO-AuNRs modified electrode (12.4 x 10(-2) cm/s), in contrast to CH-GO/ITO electrode (6 x 10(-3) cm/s). Additionally, very low K-m value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences. Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.

Item Type: Article
Uncontrolled Keywords: Partiallyreducedgrapheneoxide Gold nanorods Composite Chitosan Glucose Km Ks Flavinadeninedinucleotide(FAD) Electrochemical biosensors
Subjects: Chemistry > Analytical Chemistry
Depositing User: Dr. Rajpal Walke
Date Deposited: 06 Oct 2016 06:11
Last Modified: 06 Oct 2016 06:11

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