Dhand , Chetna and Arya, Sunil K. and Datta, Monika and Malhotra, B. D. (2008) Polyaniline–carbon nanotube composite film for cholesterol biosensor. Analytical Biochemistry , 383 (2). pp. 194-199. ISSN 1096-0309

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Nanocomposite film composed of polyaniline (PANI) and multiwalled carbon nanotubes (MWCNT), prepared electrophoretically onto indium tin oxide (ITO)-coated glass plate, was used for covalent immobilization of cholesterol oxidase (ChOx) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry. Results of linear sweep voltammetric measurements reveal that ChOx/PANI-MWCNT/ITO bioelectrode can detect cholesterol in the range of 1.29 to 12.93 mM with high sensitivity of 6800 nA mM(-1) and a fast response time of 10 s. Photometric studies for ChOx/PANI-MWCNT/ITO bioelectrode indicate that it is thermally stable LIP to 45 degrees C and has a shelf life of approximately 12 weeks when stored at 4 degrees C. The results of these studies have implications for the application of this interesting matrix (PANI-MWCNT) toward the development of other biosensors.

Item Type: Article
Additional Information: Copyright for this article belongs to M/S Elsevier.
Subjects: Biochemistry & Molecular Biology
Medical Laboratory Technology
Depositing User: Ms Neetu Chandra
Date Deposited: 02 Dec 2015 10:53
Last Modified: 02 Dec 2015 10:53
URI: http://npl.csircentral.net/id/eprint/1654

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