Prabhakar, Nirmal and Arora, Kavita and Singh, Harpal and Malhotra, Bansi D. (2008) Polyaniline based nucleic acid sensor. Journal of Physical Chemistry B, 112 (15). pp. 4808-4816. ISSN 1520-6106

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Twenty-bases long NH2-modified DNA and PNA probes specific to a pathogen (Mycobacterium tuberculosis) were covalently immobilized onto a polyaniline (PANI)/Au electrode to detect nucleic acid hybridization with complementary, one-base mismatch and noncomplementary targets within 30 s using Methylene Blue. The PNA-PANI/Au electrode exhibits improved specificity (1000 times) and detection limit (0.125 x 10(-18) M) as compared to that of the DNA-PANI/Au electrode (2.5 x 10(-18) M). These PNA-PANI/Au electrodes can be utilized for detection of hybridization with the complementary sequence in 5 min sonicated M. tuberculosis genomic DNA within I min of hybridization time. These DNA-PANI/Au and PNA-PANI/Au electrodes can be used 6-7 and 13-15 times, respectively.

Item Type: Article
Additional Information: Copyright for this article belongs to M/S American Chemical Society.
Subjects: Electrochemistry
Medical Laboratory Technology
Biochemical Research Methods
Depositing User: Ms Neetu Chandra
Date Deposited: 27 Nov 2015 10:32
Last Modified: 27 Nov 2015 10:32

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