Singal, Shobhita and Biradar, A. M. and Mulchandani, Ashok and -, Rajesh (2014) Ultrasensitive Electrochemical Immunosensor Based on Pt Nanoparticle-Graphene Composite. Applied Biochemistry and Biotechnology, 174 (3). 971-983 . ISSN 0273-2289

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Abstract

We report a protein antibody, Ab-CRP, functionalized Pt nanoparticle-decorated chemical vapor deposition (CVD)-grown graphene on glassy carbon electrode, as a bioelectrode, for the quantitative analysis of human C-reactive protein (CRP). Chemical vapor deposition was used to grow a polycrystalline graphene film on copper and was mounted over a glassy carbon electrode after copper etching through pi-pi stacking. Ab-CRP was covalently immobilized on mercaptopropionic acid (MPA)-capped Pt nanoparticles that were covalently anchored over the graphene to form a bioelectrode. The bioelectrode was characterized by scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS). A detail EIS study was conducted on the bioelectrode towards the quantitative detection of the target Ag-CRP in phosphate-buffered saline (PBS). The optimal electrical equivalent circuit that matches the impedance response of the bioelectrode was studied to delineate the biocompatibility, sensitivity, and selectivity of the bioelectrode. The bioelectrode exhibited a linear response of CRP detection in the range of 10 ng mL(-1) to 10 mu g mL(-1) with a sensitivity of 92.86 a"broken vertical bar cm(2) per decade CRP in pH 7.4 PBS.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Humana Press.
Uncontrolled Keywords:	Antibody Microscopy emical vapor deposition Voltammetry
Subjects:	Biochemistry & Molecular Biology Biotechnology & Applied Microbiology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	23 Nov 2015 10:57
Last Modified:	23 Nov 2015 10:57
URI:	http://npl.csircentral.net/id/eprint/1639

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