Ali, Md. Azahar and Srivastava, S. and Pandey, M. K. and Agrawal, Ved V. and John, R. and Malhotra, B. D. (2014) Protein-Conjugated Quantum Dots Interface: Binding Kinetics and Label-Free Lipid Detection. Analytical Chemistry, 86 (3). pp. 1710-1718. ISSN 0003-2700

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Abstract

We propose a label-free biosensor platform to investigate the binding kinetics using antigen-antibody interaction via electrochemical and surface plasmon resonance (SPR) techniques. The L-cysteine in situ capped cadmium sulfide (CdS; size < 7 nm) quantum dots (QDs) self-assembled on gold (Au) coated glass electrode have been covalently functionalized with apolipoprotein B-100 antibodies (AAB). This protein conjugated QDs-based electrode (AAB/CysCdS/Au) has been used to detect lipid (low density lipoprotein, LDL) biomolecules. The electrochemical impedimetric response of the AAB/CysCdS/Au biosensor shows higher sensitivity (32.8 k Omega mu M-1/cm(2)) in the detection range, 5-120 mg/dL. Besides this, efforts have been made to investigate the kinetics of antigen-antibody interactions at the CysCdS surface. The label-free SPR response of AAB/CysCdS/Au biosensor exhibits highly specific interaction to protein (LDL) with association constant of 33.4 kM(-1) s(-1) indicating higher affinity toward LDL biomolecules and a dissociation constant of 0.896 ms(-1). The results of these studies prove the efficacy of the CysCdS-Au platform as a high throughput compact biosensing device for investigating biomolecular interactions.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S American Chemical Society.
Subjects:	Biochemistry & Molecular Biology Medical Laboratory Technology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	04 Nov 2015 06:57
Last Modified:	04 Nov 2015 06:57
URI:	http://npl.csircentral.net/id/eprint/1485

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