Tyagi, Gunjan and Pradhan, Shrikant and Srivastava, Tapasya and Mehrotra, Ranjana (2014) Nucleic acid binding properties of allicin: Spectroscopic analysis and estimation of anti-tumor potential. BBA - Biochimica et Biophysica Acta, 1840 (1). pp. 350-356. ISSN 0006-3002

PDF - Published Version Restricted to Registered users only Download (1089Kb) | Request a copy

Abstract

Background: Allicin has received much attention due to its anti-proliferative activity and not-well elucidated underlying mechanism of action. This work focuses towards determining the cellular toxicity of allicin and understanding its interaction with nucleic acid at molecular level. Methods: MIT assay was used to assess the cell viability of A549 lung cancer cells against allicin. Fourier transform infrared (FTIR) and UV-visible spectroscopy were used to study the binding parameters of nucleic acid-allicin interaction. Results: Allicin inhibits the proliferation of cancer cells in a concentration dependent manner. FTIR spectroscopy exhibited that allicin binds preferentially to minor groove of DNA via thymine base. Analysis of tRNA allicin complex has also revealed that allicin binds primarily through nitrogenous bases. Some amount of external. binding with phosphate backbone was also observed for both DNA and RNA. UV visible spectra of both DNA allicin and RNA allicin complexes showed hypochromic shift with an estimated binding constant of 12 x 10(4) M-1 for DNA and 1.06 x 10(3) M-1 for RNA binding. No major transition from the B-form of DNA and A-form of RNA is observed after their interaction with allicin. Conclusions: The results demonstrated that allicin treatment inhibited the proliferation of A549 cells in a dosedependent manner. Biophysical outcomes are suggestive of base binding and helix contraction of nucleic acid structure upon binding with allicin. Genefal significance: The results describe cytotoxic potential of allicin and its binding properties with cellular nucleic acid, which could be helpful in deciphering the complete mechanism of cell death exerted by allicin.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Elsevier.
Uncontrolled Keywords:	Deoxyribonucleicacid Ribonucleicacid Allicin Fourier transform infrared spectroscopy MTT
Subjects:	Biochemistry & Molecular Biology Genetics & Heredity Pharmacology & Pharmacy
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	29 Oct 2015 10:59
Last Modified:	29 Oct 2015 10:59
URI:	http://npl.csircentral.net/id/eprint/1441

Actions (login required)

View Item