Ali, Md. Azahar and Reza, K. Kamil and Srivastava, Saurabh and Agrawal, Ved Varun and John, Renu and Malhotra, Bansi Dhar (2014) Lipid Lipid Interactions in Aminated Reduced Graphene Oxide Interface for Biosensing Application. LANGMUIR, 30 (14). pp. 4192-4201. ISSN 0743-7463

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Abstract

A label-free biosensor based on antiapolipoprotein B 100 functionalized-aminated reduced graphene oxide interface has been fabricated for detection of low density lipoprotein (LDL or lipid) cholesterol. The aminated reduced graphene oxide (NH2-rGO) based electrode surface is covalently functionalized with antiapolipoprotein B 100 (AAB or lipid) using EDC/NHS coupling chemistry. The lipid-lipid interactions at the NH2-rGO electrode surface have been investigated using electrochemical impedance spectroscopic technique. The structural and morphological investigations of NH2-rGO based immunosensor have been accomplished via transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, UV-visible, and electrochemical techniques. The impedimetric response of the proposed immunosensor shows excellent sensitivity (612 Omega mg(-1) dL cm(-2)), a response time of 250 s, and a low detection limit of 5 mg/dL of LDL molecules. The association, dissociation, and equilibrium rate constants for this immunoelectrode are found to be 1.66 M-1 s(-1), 0.6 s(-1), and 2.77 M-1, respectively. The long-term stability and excellent reproducibility of the proposed immunosensor indicates a suitable platform for detection of LDL or lipid molecules. This immunosensor provides an efficient platform for analysis of the antigen-antibody interactions of lipid molecules.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S American Chemical Society.
Subjects:	Biochemistry & Molecular Biology Chemistry Materials Science Medical Laboratory Technology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	19 Oct 2015 07:34
Last Modified:	19 Oct 2015 07:34
URI:	http://npl.csircentral.net/id/eprint/1376

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