Sharma, Rachna and Sinha, R. K. and Agrawal, Ved Varun (2014) Electroactive Prussian Blue Encapsulated Iron Oxide Nanostructures for Mediator-Free Cholesterol Estimation. Electroanalysis, 26 (7). pp. 1551-1559. ISSN 1040-0397

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Abstract

Iron oxide nanoparticles of size similar to 10 nm have been encapsulated into four nanometer thick shells of Prussian blue and were then electrophoretically deposited onto an indium tin oxide substrate. The immobilization of cholesterol oxidase has been done onto the nanostructured film to investigate the kinetic parameters and biosensing characteristics. The fabricated bioelectrode exhibits an electron transfer coefficient and a charge transfer rate constant of 0.45 and 45.15 s(-1), respectively. Direct electron transfer properties of the nanostructured film result in 3rd generation cholesterol biosensor. The bioelectrode exhibits high sensitivity (2.15 mAM(-1)cm(-2)), a low K-m(app) value (0.07 mM), good stability and high selectivity towards cholesterol.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Wiley-VCH Verlag.
Uncontrolled Keywords:	Iron oxide Prussian blue Kinetic analysis Direct electron transfer Cholesterol biosensor
Subjects:	Chemistry Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	01 Oct 2015 05:46
Last Modified:	01 Oct 2015 05:46
URI:	http://npl.csircentral.net/id/eprint/1265

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