Pandey, Chandra Mouli and Sumana, Gajjala and Sood, K. N. and Malhotra, B. D. (2010) Electrochemical studies of cystine modified self-assembled monolayer for Escherichia coli detection. Thin Solid Films, 519 (3). pp. 1178-1183. ISSN 0040-6090

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Abstract

An electrochemical DNA biosensor based on cystine modified self-assembled monolayer (cys-SAM) onto gold electrode (AuE) has been fabricated for Escherichia coli (E. coli) detection. This biosensing electrode has been characterized using scanning electron microscopy (SEM), FT-IR spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, this DNA biosensor can be used to detect complementary target DNA concentration in the range of 1 × 10− 6 M to 1 × 10− 20 M within 60 s of hybridization time at 25 °C and has been found to be stable for about four months when stored at 4 °C.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Cystine; Escherichia coli; Methylene blue; Self-assembled monolayer; Electrochemical DNA biosensor
Subjects:	Materials Science Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	12 Apr 2012 08:16
Last Modified:	12 Apr 2012 08:16
URI:	http://npl.csircentral.net/id/eprint/122

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