Mouli Pandey, Chandra and Sumana, Gajjala and Tiwari, Ida (2014) Copper oxide assisted cysteine hierarchical structures for immunosensor application. Applied Physics Letters, 105 (10). ISSN 0003-6951

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Abstract

The present work describes the promising electrochemical immunosensing strategy based on copper (II) assisted hierarchical cysteine structures (CuCys) varying from star to flower like morphology. The CuCys having average size of 10 mu m have been synthesised using L-Cysteine as initial precursor in presence of copper oxide under environmentally friendly conditions in aqueous medium. To delineate the synthesis mechanism, detailed structural investigations have been carried out using characterization techniques such as X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. The electrochemical behaviour of self-assembled CuCys on gold electrode shows surface controlled electrode reaction with an apparent electron transfer rate constant of 3.38 x 10(-4) cm s(-1). This innovative platform has been utilized to fabricate an immunosensor by covalently immobilizing monoclonal antibodies specific for Escherichia coli O157:H7 (E. coli). Under the optimal conditions, the fabricated immunosensor is found to be sensitive and specific for the detection of E. coli with a detection limit of 10 cfu/ml. (C) 2014 AIP Publishing LLC

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S AIP.
Subjects:	Physics
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	17 Sep 2015 11:15
Last Modified:	17 Sep 2015 11:15
URI:	http://npl.csircentral.net/id/eprint/1213

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