Mishra, Sujeet K. and Srivastava, Avanish K. and Kumar, Devendra and Rajesh, - (2014) Bio-functionalized Pt nanoparticles based electrochemical impedance immunosensor for human cardiac myoglobin. RSC Advances, 4 (41). pp. 21267-21276. ISSN 2046-2069

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We report the covalent immobilization of three-dimensional carboxyl-functionalized Pt(MPA) nanoparticles with myoglobin protein antibody by carbodiimide coupling reaction deposited onto an indium-tin-oxide-coated glass plate for the construction of a bioelectrode. This bioelectrode assembly was characterized by spectro/microscopic and electrochemical techniques. Electrochemical impedance studies of the bioelectrode showed significant changes in charge transfer resistance (R-et), predominantly in the low AC frequency region of <40 Hz, on immunoreaction with human cardiac myoglobin antigen (Ag-cMb). Ag-cMb detection in phosphate buffer exhibited a linear range of 0.01 mu g mL(-1) to 1 mu g mL(-1) with a sensitivity of 184.8 Omega cm(2) per decade

Item Type: Article
Additional Information: Copyright for this article belongs to M/S RSC.
Subjects: Chemistry
Materials Science
Depositing User: Dr. Rajpal Walke
Date Deposited: 17 Sep 2015 05:46
Last Modified: 17 Sep 2015 05:46
URI: http://npl.csircentral.net/id/eprint/1189

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