KamilReza, K. and Srivastava, Saurabh and Yadav, SurendraK and Biradar, A. M. (2014) Biofunctionalized carbon nanotubes platform for biomedical applications. Materials Letters, 126. pp. 126-130. ISSN 0167-577X

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We have fabricated the chemically functionalized multi-walled carbon nanotubes (MWCNT) electro-phoretically deposited onto indium tin oxide for urea detection. Urease and glutamate dehydrogenase are attached to MWCNT/ITO electrode surface covalently using ethyl-dimethylaminopropyl-carbodiimide and N-hydroxysuccinimide coupling chemistry. Excellent electrochemical properties of MWCNT and covalent functionalization of dual enzyme improve biosensor stability and sensitivity. The electrochemical response studies of the proposed biosensor exhibits wide detection range (0.83-24.9 mM), low detection limit (1.3 mM) and higher sensitivity of 4.67 mu A mM(-1) cm(-2). The low magnitude (0.274 mM) of the apparent Michaelis-Menten constant (Km) value indicates high enzymatic affinity of Urs-GLDH enzymes on MWCNT/ITO electrode surface.

Item Type: Article
Additional Information: Copyright for this article belongs to M/S Elsevier.
Subjects: Materials Science
Depositing User: Dr. Rajpal Walke
Date Deposited: 17 Sep 2015 05:42
Last Modified: 17 Sep 2015 05:42
URI: http://npl.csircentral.net/id/eprint/1187

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