Azahar Ali, Md. and Srivastava, Saurabh and Mondal, Kunal and M Chavhan, Pandurang and John, Renu and Agrawal, Ved V. and Sharma, Ashutosh and Malhotra, Bansi D. (2014) A surface functionalized nanoporous titania. integrated microfluidic biochip. Nanoscale, 6 (22). pp. 13958-13969. ISSN 2040-3364

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We present a novel and efficient nanoporous microfluidic biochip consisting of a functionalized chitosan/anatase titanium dioxide nanoparticles (antTiO(2)-CH) electrode integrated in a polydimethylsiloxane (PDMS) microchannel assembly. The electrode surface can be enzyme functionalized depending on the application. We studied in detail cholesterol sensing using the cholesterol esterase (ChEt) and cholesterol oxidase (Ch0x) functionalized chitosan supported mesoporous antTiO(2)-CH microfluidic electrode. The available functional groups present in the nanoporous antTiO(2)-CH surface in this microfluidic biochip can play an important rote for enzyme functionalization, which has been quantified by the X-ray photoelectron spectroscopic technique. The Brunauer-Emmett-Teller (BET) studies are used to quantify the specific surface area and nanopore size distribution of titania nanoparticles with and without chitosan. Point defects in antTiO(2) can increase the heterogeneous electron transfer constant between the electrode and enzyme active sites, resulting in improved electrochemical behaviour of the microfluidic biochip. The impedimetric response of the nanoporous microfluidic biochip (ChEt-ChOx/antTiO(2)-CH) shows a high sensitivity of 6.77 k Omega (mg dl(-1))(-1) in the range of 2-500 mg dl(-1), a low detection limit of 0.2 mg dl(-1), a low Michaelis-Menten constant of 1.3 mg dl(-1) and a high selectivity. This impedimetric microsystem has enormous potential for clinical diagnostics applications

Item Type: Article
Additional Information: Copyright for this article belongs to M/S Royal Society of Chemistry.
Subjects: Chemistry
Materials Science
Depositing User: Dr. Rajpal Walke
Date Deposited: 08 Sep 2015 10:30
Last Modified: 08 Sep 2015 10:30

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