Singh, Jay and Roychoudhury, Appan and Srivastava, Manish and Solanki, Pratima R. and Lee, Dong Won and Lee, Seung Hee and Malhotra, B. D. (2014) A dual enzyme functionalized nanostructured thulium oxide based interface for biomedical application. Nanoscale, 6 (2). pp. 1195-1208. ISSN 2040-3364

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Abstract

In this paper, we present results of the studies related to fabrication of a rare earth metal oxide based efficient biosensor using an interface based on hydrothermahy prepared nanostructured thulium oxide (n-Tm2O3). A cohoidal solution of prepared nanorods has been electrophoreticay deposited (EPD) onto an indium-tin-oxide (ITO) glass substrate. The n-Tm2O3 nanorods are found to provide improved sensing characteristics to the electrode interface in terms of electroactive surface area, diffusion coefficient, charge transfer rate constant and electron transfer kinetics. The structural and morphological studies of n-Tm2O3 nanorods have been carried out by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission eLectron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopic techniques. This interfacial platform has been used for fabrication of a total cholesterol biosensor by immobilizing cholesterol esterase (ChEt) and choLesterol oxidase (ChOx) onto a Tm2O3 nanostructured surface. The results of response studies of the fabricated ChEt-ChOx/n-Tm2O3/ITO bioeLectrode show a broad Linear range of 8-400 mg dL(-1), detection Limit of 19.78 mg (dL cm(-2))(-1), and high sensitivity of 0.9245 RA (mg per dL cm(-2))(-1) with a response time of 40 s. Further, this bioeLectrode has been utiLized for estimation of total cholesterol with negligible interference (3%) from analytes present in human serum samples. The utilization of this n-Tm2O3 modified electrode for enzyme-based biosensor analysis offers an efficient strategy and a novel interface for application of the rare earth metal oxide materials in the field of electrochemical sensors and bioelectronic devices

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S RSC.
Subjects:	Chemistry Materials Science Medical Laboratory Technology Physics
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	08 Sep 2015 11:09
Last Modified:	08 Sep 2015 11:09
URI:	http://npl.csircentral.net/id/eprint/1147

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