Prabhakar, Nirmal and Sumana, G. and Arora, Kavita and Singh, Harpal and Malhotra, B. D. (2008) Improved electrochemical nucleic acid biosensor based on polyaniline-polyvinyl sulphonate. Electrochimica Acta, 53. pp. 4344-4350. ISSN 0013-4686

PDF - Published Version Restricted to Registered users only Download (731Kb) | Request a copy

Abstract

DNA biosensor based on polyaniline (PANI)-polyvinyl sulphonate (PVS) has been fabricated using electrochemical entrapment technique for detection of organophosphorus pesticides (chlorpyrifos and malathion). These double stranded calf thymus DNA (dsCT-DNA) entrapped PANI-PVS/indium-tin-oxide (ITO) bioelectrodes have been characterized using square wave voltammetry (SWV), Fourier transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM) and electrochemical impedance techniques, respectively. These dsCT-DNA entrapped PANI-PVS/ITO bioelectrodes have been found to have response time of 30 s, stability of about 6 months and detection limit for chlorpyrifos and malathion as 0.5 ppb and 0.01 ppm, respectively.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Subjects:	Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Biochemical Research Methods
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	10 Mar 2015 10:31
Last Modified:	10 Mar 2015 10:31
URI:	http://npl.csircentral.net/id/eprint/1073

Actions (login required)

View Item