Prabhakar, Nirmal and Sumana, G. and Arora, Kavita and Singh, Harpal and Malhotra, B. D. (2008) Improved electrochemical nucleic acid biosensor based on polyaniline-polyvinyl sulphonate. Electrochimica Acta, 53. pp. 4344-4350. ISSN 0013-4686

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DNA biosensor based on polyaniline (PANI)-polyvinyl sulphonate (PVS) has been fabricated using electrochemical entrapment technique for detection of organophosphorus pesticides (chlorpyrifos and malathion). These double stranded calf thymus DNA (dsCT-DNA) entrapped PANI-PVS/indium-tin-oxide (ITO) bioelectrodes have been characterized using square wave voltammetry (SWV), Fourier transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM) and electrochemical impedance techniques, respectively. These dsCT-DNA entrapped PANI-PVS/ITO bioelectrodes have been found to have response time of 30 s, stability of about 6 months and detection limit for chlorpyrifos and malathion as 0.5 ppb and 0.01 ppm, respectively.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Elsevier B.V.
Subjects: Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Biochemical Research Methods
Depositing User: Ms Neetu Chandra
Date Deposited: 10 Mar 2015 10:31
Last Modified: 10 Mar 2015 10:31

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