Pandey, P. and Singh , S. P. and Arya , S. K. and Sharma , A. and Datta, M. and Malhotra , B. D. (2008) Gold nanoparticle-polyaniline composite films for glucose sensing. Journal of Nanoscience and Nanotechnology, 8 (6). 3158-63-3163. ISSN 1533-4880

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Abstract

Gold nanoparticles (AuNPs) have been self-assembled onto electrochemically deposited polyaniline (PANI) films on indium-tin-oxide (ITO) coated glass plates to fabricate glucose biosensor. The covalent immobilization of glucose oxidase (GOx) in the near vicinity of gold nanoparticles has been obtained using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS), chemistry between amino groups of PANI and COOH groups of GOx. These AuNPs-PANI/ ITO and GOx/AuNPs-PANI/ITO composite films have been characterized using Fourier transform infra red (FTIR) and cyclic voltammetry (CV) techniques, respectively. The fast electron transfer from the modified PANI surface to electrode is indicated by the observed increase in amperometric response current of these GOx/AuNPs-PANI/ITO bioelectrodes. These GOx/AuNPs-PANI/ITO bioelectrodes exhibit response time of 10 s, linearity from 50 to 300 mg/dl and show value of apparent Michaelis-Menten constant (Km(app)) of 2.2 mM.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s American Scientific Publishers.
Uncontrolled Keywords:	Gold Nanoparticles, Polyaniline, Composite, Glucose Sensing, Biosensor
Subjects:	Nanoscience/ Nanotechnology Physical Chemistry/Chemical Physics Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	28 Oct 2014 07:38
Last Modified:	28 Oct 2014 07:38
URI:	http://npl.csircentral.net/id/eprint/1038

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